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With a 2:1 mixture of methanol and acetone, and then dried. Plaques were visualized with a cocktail of polyclonal antibodies to gD, gB, and gC by black plaque assay (27, 57) using horseradish peroxidase-conjugated protein A, followed by addition of the substrate 4-chloro-1-naphthol. The neutralization titer was expressed as the dilution of serum that reduced the number of plaques by 50 . Two assay
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