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Rs after DNA binding. The FokI dimer catalyses formation of a double-strand break in the DNA spacer region between the two TALEN target sites. We created an analogous architecture using Bat1 to confer DNA binding specificity and compared its activity in an in vitro cleavage assay against the corresponding TALEN (dTALEBat1mimic -FokI; sequences given in Supplementary Figure S5). As target DNA we us
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