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Of exocytosis. This inhibitor of exocytosis was designed to target NSF, a critical component of the exocytic machinery (16?8). We developed a fusion polypeptide with two domains, a TAT domain, which enters cells through macropinocytosis, and an NSF domain that inhibits NSF. This fusion peptide, designated TAT-NSF222, inhibits NSF ATPase activity, decreases endothelial exocytosis, and blocks inflam
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